Part:BBa_K1539001:Experience

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Applications of BBa_K1539001

BBa_K1539001 was created using devices BBa_K1539021 [[1]] and BBa_K1539055 [[2]] to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650 [[3]]. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [4]). Our raw data is summarized below:

"High Expression Combination" - Primers PH1/RH1:

File:140821 DMC mCherry PH2-RH2.pdf

Negative Control:

File:140821 DMC mCherry Promoterless.pdf

User Reviews

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